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Description
Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24795 shows an immuno EM image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3a-c from J Cell Biol. 1999 May 31;145(5):1009-26.
Technical Details
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
Biological Sources
- NCBI Organism Classification
- Xenopus laevis
- (African clawed frog)
- Cell Type
- keratocyte
- Cellular Component
- lamellipodium
- actin cytoskeleton
Biological Context
- Biological Process
- cellular macromolecule localization
Attribution
- Names
- Tatyana M. Svitkina
- Gary G. Borisy
- Published
- J Cell Biol. 1999 May 31;145(5):1009-26.
- Pubmed
- 10352018
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- fluorescence microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- Fluorescein (FITC)
- Tetramethyl rhodamine (TRITC)
- phalloidin
- primary antibody plus labeled secondary antibody
- Processing History
- unprocessed raw data
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- methanol fixed tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 699px | 0.126μm |
| Y | 1044px | 0.126μm |
*CIL – Cell Image Library accession number. Please use this to reference an image.