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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24730*  Cite 
Description

Mouse 3T3 fibroblast immunostained for the nucleolar protein nucleostemin (left, green) and fibrillarin (right, red) marking nucleolar fibrillar centers.

Technical Details

Data were collected using a Leica DMIRB microscope equipped with a 100x objective (N.A. 1.4) and appropriate filter sets, and images captured using a Quantix 57 CCD camera (Roper Scientific Photometrics). For high resolution spatial mapping, three-dimensional optical stacks (containing 21 consecutive 0.25 micron slices) were captured using a PIFOC microscope focusing drive (Polytec PI). Images were dark current subtracted, intensity scaled, and in some cases, z-stacks deconvolved using exhaustive photon reassignment. Shown is a midplane of a deconvolved stack. See Fig 2EF in Politz et al., 2005 Mol Biol Cell 16:3401-3410.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
3T3
Cellular Component
nucleolus
Attribution
Names
Joan Politz
Published
Politz et al., 2005 Mol Biol Cell 16:3401-3410
Pubmed
15857956
Link
Molecular Biology of the Cell
OTHER
Ilvin Polena
Ian Trask
David Bazett-Jones
Thoru Pederson
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24730
Archival Resource Key (ARK)
ark:/b7295/w9cil24730
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
Fluorescein (FITC)
Tetramethyl rhodamine (TRITC)
Processing History
multipanel montage
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 468px ——
Y 240px ——