The Cell: An Image Library

Licensing

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CIL:239^*

Description

The image depicts a live neutrophil cell showing the level of activation of the signaling protein Cdc42, a member of the Rho family of small guanosine triphosphatase (GTPase) proteins which regulates multiple cell functions, including motility, proliferation, apoptosis, and cell morphology. Here, the fluorescence level is related to protein conformational changes and interactions, with warmer colors indicating higher levels of activation.

Technical Details

See P. Nalbant et al 2004. Activation of endogenous Cdc42 visualized in living cells. Science 305:1615-1619.

Biological Sources

NCBI Organism Classification: Mus musculus; (house mouse)
Cell Type: fibroblast
Cell Line: MEF
Cellular Component: cdc2

Attribution

Name: Klaus Hahn
Link: NIGMS Image Library

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in protein conformation

Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1295px	——
Y	1604px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.