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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23607*  Cite 
Description

Nuclear envelope and spindle dynamics when the nucleus moves into the neck of kar9delta mutant cells arrested in S phase. Movie plays at 100x real time and is movie S6 in Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATalpha NUP133-tdimer2::k.l.URA3 kar9delta::hygB ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) expressing GFP-tubulin (GFP-TUB1, to mark spindle and microtubules, top panel, green in bottom panel merge) and RFP-NUP133 (to mark nuclear envelope, middle panel, red in bottom panel merge) were arrested in hydroxyurea and mounted on agarose pads for microscopy. Images were captured on an Olympus Bmax-60F microscope equipped with a 1.35NA 100× UPlanApo objective, spinning disc Confocal Scanner Unit (CSU10), Picarro Cyan laser (488 nm) and Cobolt Jive laser (561 nm), and a Stanford Photonics XR-Mega10 ICCD camera, by using QED software (Media Cybernetics). Image analysis was performed by using ImageJ. Timelapse images were captured at 20-s intervals for 10 min and each image in the movie represents a composite of 9 planes separated by 500 nm.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cell Line
kar9delta
Cellular Component
spindle pole body
cytoplasmic microtubule
microtubule
nuclear pore
nuclear membrane
Attribution
Names
Jeffrey K. Moore
David Sept
John A. Cooper
Published
Proc Natl Acad Sci. 2009. 106: 5147-5152.
Pubmed
19279216
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL23607
Archival Resource Key (ARK)
ark:/b7295/w9cil23607
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
DsRed2FP
Processing History
each frame is a composite of 9 planes
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 450px ——
Y 744px ——
Time 20 seconds 30