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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23599*  Cite 

Spindle oscillation in wild-type cells arrested in S phase. In wild-type cells, the spindle moves in both directions through the bud neck; these movements coincide with lateral sliding of a cytoplasmic microtubule along the cell cortex. These events require dynein-dynactin function since nip100delta mutants display frequent contacts between microtubule ends and the cell cortex, but no spindle movements or microtubule sliding events were observed (Movie S2, CIL# 23603). Movie plays at 50x real time and is Movie S1 in Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATalpha ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) expressing GFP-tubulin (GFP-TUB1) were arrested in hydroxyurea and mounted on agarose pads for microscopy. Images were captured on an Olympus Bmax-60F microscope equipped with a 1.35NA 100× UPlanApo objective, spinning disc Confocal Scanner Unit (CSU10), Picarro Cyan laser (488 nm), and a Stanford Photonics XR-Mega10 ICCD camera, by using QED software (Media Cybernetics). Image analysis was performed by using ImageJ. Timelapse images were captured at 10-s intervals for 15 min and each image in the movie represents a composite of 9 planes separated by 500 nm.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cellular Component
spindle pole body
cytoplasmic microtubule
Biological Context
Biological Process
microtubule-based movement
Molecular Function
GTPase activity
structural constituent of cytoskeleton
Jeffrey K. Moore
David Sept
John A. Cooper
Proc Natl Acad Sci. 2009. 106: 5147-5152.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
each frame is a composite of 9 planes
Data Qualifiers
processed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 435px ——
Y 276px ——
Time 10 seconds 87