Association of plectin with MTs. Electron microscopy
of gelsolin-treated REF-52 cells after immunogold (10 nm) labeling for Tyr-tubulin. Plectin forms bridges between microtubules (MTs) and intermediate filaments (IFs). MTs composed predominantly of Tyr-tubulin interact with IFs via plectin. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 5b from J Cell Biol. 1996 Nov;135(4):991-1007.