Association of plectin with MTs. Electron microscopy of gelsolin-treated REF-52 cells after immunogold (10 nm) labeling for plectin. Plectin forms bridges between microtubules and intermediate filaments. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 5a from J Cell Biol. 1996 Nov;135(4):991-1007.
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