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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23045*  Cite 
Description

Preferential distribution of plectin in peripheral regions. REF-52 cells were treated with nocodazole to depolymerize MTs and induce collapse of most IFs to the cell center. Electron microscopy of peripheral cell regions after gelsolin treatment and immunogold (10 nm) labeling for plectin. Short intermediate filaments at the periphery have numerous plectin sidearms. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
fibroblast
Cell Line
REF-52
Cellular Component
intermediate filament
plectin
Biological Context
Biological Process
nocadazole treatment
gelsolin treatment
Attribution
Names
Tatyana M. Svitkina
Alexander B. Verkhovsky,
Gary G. Borisy
Published
J Cell Biol. 1996 Nov;135(4):991-1007
Pubmed
8922382
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL23045
Archival Resource Key (ARK)
ark:/b7295/w9cil23045
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
tannic acid
critical_point dried specimen
Relation To Intact Cell
platinum replica
Dimensions
Spatial Axis Image Size Pixel Size
X 3281px ——
Y 3224px ——