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Description
Plectin sidearms on intermediate filaments labeled with plectin immuno-gold (10 nm) in REF-52 cells with actin removed by gelsolin treatment. Untreated control appears as CIL: 234044. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.
Biological Sources
- NCBI Organism Classification
- Rattus
- (Old World rats)
- Cell Type
- fibroblast
- Cell Line
- REF-52
- Cellular Component
- intermediate filament
- plectin
Biological Context
- Biological Process
- gelsolin treatment
Attribution
- Names
- Tatyana M. Svitkina
- Alexander B. Verkhovsky,
- Gary G. Borisy
- Published
- J Cell Biol. 1996 Nov;135(4):991-1007
- Pubmed
- 8922382
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- transmission electron microscopy (TEM)
- Parameters Imaged
- elastic scattering of electrons
- Source of Contrast
- differences in deposition of metal shadow
- Visualization Methods
- uranyl salt
- shadowing and plating
- gold
- primary antibody plus labeled secondary antibody
- immunogold
- Processing History
- unprocessed raw data
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- tannic acid
- critical_point dried specimen
- Relation To Intact Cell
- platinum replica
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 3102px | —— |
| Y | 2229px | —— |
*CIL – Cell Image Library accession number. Please use this to reference an image.