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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23043*  Cite 
Description

Plectin sidearms on intermediate filaments labeled with plectin immuno-gold (10 nm) in REF-52 cells with actin removed by gelsolin treatment. Untreated control appears as CIL: 234044. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
fibroblast
Cell Line
REF-52
Cellular Component
intermediate filament
plectin
Biological Context
Biological Process
gelsolin treatment
Attribution
Names
Tatyana M. Svitkina
Alexander B. Verkhovsky,
Gary G. Borisy
Published
J Cell Biol. 1996 Nov;135(4):991-1007
Pubmed
8922382
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL23043
Archival Resource Key (ARK)
ark:/b7295/w9cil23043
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
tannic acid
critical_point dried specimen
Relation To Intact Cell
platinum replica
Dimensions
Spatial Axis Image Size Pixel Size
X 3102px ——
Y 2229px ——