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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:21697*  Cite 
Description

Quick-freeze deep-etch image of a fracture through the radial canal of the contractile vacuole. This technique presents a picture of a confusing array of non-etchable material surrounding a collecting canal. Lumens of smooth spongiome tubules can be observed. One gets the impression that these tubules are not entirely smooth but that they do have subunits of some sort on their cytosolic surfaces. A few segments of decorated tubules with helically wound subunits on their cytosolic surfaces are visible at the periphery of this radial arm. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:13119) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuole
contractile vacuolar membrane
Biological Context
Biological Process
contractile vacuole organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL21697
Archival Resource Key (ARK)
ark:/b7295/w9cil21697
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
elevation
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
freeze_fracture/freeze_etch
shadowing and plating
recorded image
film
Print of negative scanned for Photoshop
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 3000px ——
Y 3696px ——