The Cell: An Image Library

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

Comments:

Add to Photobox

CIL:2^*

Description

The distribution of the mitochondria (labeled with antibody to hsp70) in relation to intermediate filaments (labeled with vimentin antibody) 8 hours after release from serum starvation in methanol-fixed 3T3 fibroblasts. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 100X oil/1.3 NA Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20, Em 515-565 BP, dichroic 510 2. Ex 560/40, Em 630/60, dichroic 595

Biological Sources

NCBI Organism Classification: Mus musculus; (house mouse)
Cell Type: fibroblast
Cellular Component: type III intermediate filament; mitochondrion

Biological Context

Biological Process: intermediate filament-based process; mitochondrion morphogenesis
Molecular Function: cytoplasm organization; ATP biosynthetic process

Attribution

Names: Linda Parysek; Trudy Aebig

Imaging

Image Type: microscopy
Imaging Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: localized accumulation of fluorescent probe
Visualization Methods: Alexa Fluor 594; Alexa Fluor 488; primary antibody plus labeled secondary antibody
Processing History: unprocessed raw data

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1300px	104nm
Y	1030px	104μm

*CIL – Cell Image Library accession number. Please use this to reference an image.