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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:19326*  Cite 

Acanthamoeba castellani is a free living amoebae found in soil and water and can be an opportunistic pathogen as well. They have been diagnosed as the causative agent associated with keratitis, an infection of the eye, in humans. Other opportunistic pathogens are Naegleria and Balamuthia which have been associated with amebic encephalitis. Acanthamoeba and Naegleria feed by phagocytosis while Balamuthia feed by inserting the tips of their pseudopodia into cells. The other members of the video group, Naegleria and Balamuthia, are linked to this movie. The cells were grown in axenic media. Then they were photographed with a Canon Power Shot G3 with a setting at ISO 50 with an optical microlens at 4X and the quality set at RAW. The camera was attached to the phototube of the microscope with a 3X lens and photographed in phase contrast with a 100X oil immersion objective. Live amoebae were placed in a chamber prepared by inverting a coverslip with/or without cultured mammalian cells onto a 3X1 inch microscope slide with a thin rim of Vaseline; melted paraffin was brushed around the edge of the coverslip to seal it to the slide. Such cultures could be maintained for several days at room temperature.

Biological Sources
NCBI Organism Classification
Acanthamoeba castellanii
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Amoeboid Protist
Cellular Component
Thelma Dunnebacke
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
in-focus phase contrast
Image Mode
phase contrast microscopy
Parameters Imaged
boundaries between regions with different refractive index
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
natural boundaries
Processing History
living tissue
recorded image
charge coupled device (CCD)
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
living tissue
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 320px ——
Y 240px ——