Licensing

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Description

Micrograph of a semi-thick (1-2 µm) section of an infected maize leaf, with silver-enhanced immunogold labeling, that shows dark staining of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates in phloem tissue (asterisk). The section was counter stained with toluidine blue. CIL:12591, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Additional related images from this contributor are available in the Library.

Biological Sources

NCBI Organism Classification
Spiroplasma kunkelii
Zea mays
(corn)
Cell Type
plant cell
CL:0000399
prokaryotic cell
Cellular Component
plant-type cell wall

Biological Context

Biological Process
pathogenesis

Attribution

Names
El-Desouky Ammar
Saskia A. Hogenhout

Grouping

This image is part of a group.

Imaging

Image Type
bright-field microscopy
Imaging Mode
widefield illumination
Parameters Imaged
absorption of illumination
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
toluidine blue
gold
Data Qualifiers
processed data

Dimensions

Spatial Axis Image Size Pixel Size
X 568px ——
Y 976px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.