Licensing
Description
Micrograph of a semi-thick (1-2 µm) section of an infected maize leaf, with silver-enhanced immunogold labeling, that shows dark staining of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates in phloem tissue (asterisk). The section was counter stained with toluidine blue. CIL:12591, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Additional related images from this contributor are available in the Library.
Biological Sources
- NCBI Organism Classification
- Spiroplasma kunkelii
- Zea mays
- (corn)
- Cell Type
- plant cell
- CL:0000399
- prokaryotic cell
- Cellular Component
- plant-type cell wall
Biological Context
- Biological Process
- pathogenesis
Attribution
- Names
- El-Desouky Ammar
- Saskia A. Hogenhout
Grouping
This image is part of a group.Imaging
- Image Type
- bright-field microscopy
- Imaging Mode
- widefield illumination
- Parameters Imaged
- absorption of illumination
- Source of Contrast
- differences in adsorption or binding of stain
- Visualization Methods
- toluidine blue
- gold
- Data Qualifiers
- processed data
Sample Preparation
- Methods
- tissue in epoxy resin embedment
- glutaraldehyde fixed tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 568px | —— |
| Y | 976px | —— |