The Cell: An Image Library

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CIL:191^*

Description

The image shows mitotic metaphase (upper) and anaphase (lower) in Drosophila tissue culture cells immunostained for the microtubule severing protein katanin (green), microtubules (red) and kinetochores/chromosomes (blue). Katanin targets to chromosomes during both metaphase and anaphase and is responsible for inducing the depolymerization of attached microtubule plus-ends.

Technical Details

See D. Zhang et al. 2007 Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes. J Cell Biol 177, 231-242.

Biological Sources

NCBI Organism Classification: Drosophila melanogaster; (Common fruit fly)
Cell Line: S2
Cellular Component: spindle; nuclear chromosome

Biological Context

Biological Process: mitosis

Attribution

Names: David Sharp; Dong Zhang; Gregory Rogers; Daniel Buster

Imaging

Image Type: recorded image
Imaging Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Processing History: montage

Data Qualifiers: processed data

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	700px	——
Y	523px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.