Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:188^* Cite

Description

The image shows a nucleus isolated from Arabidopsis thaliana immunostained for the HDA6 histone deacetylase (red). DNA is stained blue. HDA6 concentrates in the nucleolus (top center), where ribosomal RNA genes reside.

Technical Details

See K. Earley et al. 2006 Erasure of histone acetylation by Arabidopsis HDA6 mediates large-scale gene silencing in nucleolar dominance. Genes Dev. 20:1283-1293.

Biological Sources

NCBI Organism Classification: Arabidopsis thaliana
Cell Type: root
Cellular Component: nucleus

Biological Context

Biological Process: regulation of gene expression, epigenetic; nucleus organization
Molecular Function: histone deacetylase activity

Attribution

Names: Olga Pontes; Craig Pikaard
Link: NIGMS Biomedical Beat

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL188
Archival Resource Key (ARK): ark:/b7295/w9cil188

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: Tetramethyl rhodamine (TRITC); 4',6-diamidino-2-phenylindole (DAPI)
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	965px	——
Y	965px	——