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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:17893*  Cite 
Description

Conchophthirus was deciliated by calcium ion shock followed by shearing through a 22 gauge needle. This revealed the location of the deciliated basal bodies and the cell's surface architecture. In this image of the suture line where the left and right side rows of basal bodies converge, one can observe the distribution of the ciliature, and at the suture line the location of the deciliated caudal basal bodies to which 10-20 long caudal cilia are attached. These cilia seem to act as a rudder to help guide the organism as the active beating cilia of locomotor cilia propagate the organism through the media. Also note that, when zoomed in, in this preparation the clathrin coated pits, which are normally an invagination into the cytoplasm have everted following the deciliation procedure, to form a small evagination anterior to the stubb of each deciliated basal body. For more information see: Antipa, G. A. and Small, E. B. 1971. A redescription of Conchophthirus curtus Engelmann, 1862 (Protozoa, Ciliatea). J. Protozool. 18:491-503. This micrograph was taken in 1968 by G. Antipa on a Cambridge Mark IIA operating at 20kV. The negative magnification is 1912X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis.

Biological Sources
NCBI Organism Classification
uncultured scuticociliate
Conchophthirus curtus
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cilium
caudal cilia
coated pit
Biological Context
Biological Process
cortical cytoskeleton organization
Attribution
Names
Gregory Antipa (San Francisco State University)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL17893
Archival Resource Key (ARK)
ark:/b7295/w9cil17893
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
illumination by electrons
secondary_electron imaging
Parameters Imaged
illumination by electrons
secondary electron generation
secondary_electron imaging
Source of Contrast
detection of electrons
Visualization Methods
Deciliation
Processing History
recorded image
film
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
shadowing and plating
chemically fixed tissue
Parducz fixative
lyophilized specimen
Au/Pd metal sputter-coat
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 6125px 8.3nm
Y 5192px 8.3nm