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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:17446*  Cite 

In a cell treated with trifluoperizine (TFP), all stages of endocytosis and movement to the early endosomes and out of the early endosomes can be found. These steps begin at the parasomal sac and ending with an uncoated carrier vesicles ready to pass into the cell’s interior. This micrograph is of the oral region. TEM taken on 10/9/87 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. Adapted with permission from Fig. 15 published in J. Cell Sci. 101:449-461, 1992. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12616) is in the library and available for quantitative analysis. Following trifluoperizine (TFP) treatment, standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
cortical cytoskeleton
clathrin coat of endocytic vesicle
Richard Allen (University of Hawaii)
J. Cell Sci. 101:449-461, 1992
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
horseradish peroxidase
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2304px ——
Y 2454px ——