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CIL:17443*  Cite 

An early endosome in planar view that contains HRP-reaction product. This cell was exposed to HRP for 6 min. and then fixed. This early endosome had two small coated evaginations arising from its rim and also had two large HRP-containing uncoated vesicles docked next to it presumably ready to fuse with it (for this picture and a serial section showing these two vesicles and the second evagination see Fig. 14C in Allen, Schroeder and Fok, J. Cell Sci. 101:449-461, 1992). Mitochondria have an endogenous peroxidase activity. This image was adapted with permission from Fig. 14. TEM taken on 10/20/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12617) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
early endosome
Biological Context
Biological Process
cortical cytoskeleton organization
endosome organization
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2430px ——
Y 2139px ——