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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:17441*  Cite 
Description

Paramecium multimicronucleatum is covered with cilia which cilia arise from regular surface depressions roughly rectangular in shape. This image is adapted with permission from Fig. 1 in J. Cell Biol. 49:1-20, 1971. The plasma membrane covers the exterior of the cell and this is closely attached to a mosaic of alveolar sacs internally which in this micrograph are expanded. Borders of adjacent alveolar sacs form septa along each row of cilia and sometimes laterally as well. Septa do not follow the crests of the ridges. The ridges of the rectangular depressions at their tips are filled internally with a granulo-fibrillar material. Trichocyst tips penetrate into the transverse margins along each row of cilia. In this micrograph the trichocysts have been expelled and in a few cases only their membranous casings remain. Collidine buffered fixative. TEM taken on 12/20/68 by R. Allen with Philips 300 operating at 60kV. Neg. 16,000X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12029) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
plasma membrane
axoneme
cell cortex
alveoli
Attribution
Names
Richard Allen (University of Hawaii)
Published
J. Cell Biol. 49:1-20, 1971
Pubmed
4102004
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL17441
Archival Resource Key (ARK)
ark:/b7295/w9cil17441
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2793px ——
Y 2154px ——


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