The Cell: An Image Library

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

Comments:

Add to Photobox

CIL:168^*

Description

The image shows a nucleus extracted from the brain of an old (29 month) rat and immuno-stained for tubulin (green)and nuclear pore complexes (red). DNA was stained with DAPI (blue). The intra-nuclear accumulation of tubulin and other cytoplasmic proteins is a property of aging cells apparently caused by deterioration of nuclear pore complexes.

Technical Details

See D'Angelo et al. 2009 Cell 136:284-295.

Biological Sources

NCBI Organism Classification: Rattus norvegicus; (Brown Rat)
Cell Type: neuron
Cellular Component: tubulin complex; nuclear envelope

Biological Context

Biological Process: cell aging

Attribution

Names: Maximilliano D'Angelo; Martin Hetzer
Published: D'Angelo et al. 2009 Cell 136:284-295.
Link: BioMedical Beat

Imaging

Image Type: charge coupled device (CCD)
Imaging Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: Fluorescein (FITC); 4',6-diamidino-2-phenylindole (DAPI); Tetramethyl rhodamine (TRITC)
Processing History: montage

Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	959px	——
Y	1272px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.