Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:1584^* Cite

Description

Cultured chicken embryonic cardiomyocyte fixed and stained for alpha-actinin using a monoclonal antibody and Alexa-546 as a secondary fluorescent antibody. Imaged by widefield epifluorescence on a Zeiss Axioplan microscope with a 63x objective. Image taken with a Hamamatsu Orca-ER CCD camera. Scale bar is 6um.

Biological Sources

NCBI Organism Classification: Gallus gallus gallus
Cell Type: early embryonic cell; cardiac muscle cell
Cellular Component: striated muscle dense body; alpha-actinin; Z-line component

Biological Context

Biological Process: striated muscle contraction; cytoskeleton organization
Molecular Function: actin binding; actin cross-linking

Attribution

Names: Caroline McKeown

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL1584
Archival Resource Key (ARK): ark:/b7295/w9cil1584

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; distribution of a specific protein
Visualization Methods: Alexa Fluor 546

Sample Preparation

Methods: formaldehyde fixed tissue; detergent permeabilized

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	0.067µm
Y	1024px	0.067µm