Untreated 16-cell purple urchin embryo with EMTB-3G (microtubules) in shown cyan and mC-H2B (chromatin) in yellow.). The video shows normal correlation between aster development, furrowing, and the extent of separation of sister chromatids. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. The video corresponds to the first segment of video 5 and Fig. S3A in J Cell Biol. 2009 Dec 14;187(6):831-45. Note this video is the control video for an embryo treated with trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells that is also part of this image group.
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