Alternate header for print version


Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:15791*  Cite 
Description

One of eight cells in a slightly compressed sand dollar embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). The embryo was treated with 20 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells at time 00:00, before nuclear envelope breakdown. After an exceptionally long metaphase (>20 min) with no long astral microtubules, and during which one half of the spindle bifurcated, this cell entered anaphase approximately 26 min after TSA addition. Some astral microtubules regrew in anaphase, but none approach within 10 µm of the cortex. Sand dollar embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. Video corresponds to the fourth segment of Video 4 and Figure S4B of J Cell Biol. 2009 Dec 14;187(6):831-45.

Biological Sources
NCBI Organism Classification
Dendraster excentricus
Cell Line
Embryo
Cellular Component
microtubule
ensconsin
astral microtubule
spindle
Biological Context
Biological Process
mitotic metaphase
mitotic anaphase
TSA treatement
Attribution
Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL15791
Archival Resource Key (ARK)
ark:/b7295/w9cil15791
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.25µm
Y 512px 0.25µm
Time 10 seconds 319