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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:15788*  Cite 
Description

16-cell purple urchin embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). The embryo was treated with 20 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells, at time 00:00. Within minutes of TSA addition in metaphase, asters were reduced to nearly nothing (04:00). Despite reduction in spindle length, cells enter anaphase (07:20) and complete cytokinesis (11:20–16:10). No microtubules connecting the mitotic apparatus to the cortex are visible. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens at room temperature. Corresponds to Figure 4A and first segment of video 4 in J Cell Biol. 2009 Dec 14;187(6):831-45.

Biological Sources
NCBI Organism Classification
Strongylocentrotus purpuratus
Cell Line
Embryo
Cellular Component
microtubule
ensconsin
astral microtubule
spindle
Biological Context
Biological Process
mitotic metaphase
mitotic anaphase
cytokinesis after mitosis
trichostatin A treatment
Attribution
Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL15788
Archival Resource Key (ARK)
ark:/b7295/w9cil15788
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.21µm
Y 512px 0.21µm
Time 10 seconds 134