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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:15784*  Cite 
Description

6-cell sand dollar embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). 10 µM nocodazole was added at time 00:00, at which time three of four macromeres have entered anaphase; the east cell enters anaphase shortly thereafter (03:00). In all cells, numerous astral microtubules, pointing in all directions, persist >5 min after nocodazole addition. Stable microtubules may approach the cortex in west and south cells (03:00), but none can be seen to do so in north and east cells; all commence furrowing at a normal time and complete cytokinesis. Note that in all cases, stable microtubules become brighter because EMTB-3G liberated by disassembly becomes available for binding. Sand dollar embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C, which is tolerated well. Movie corresponds to Fig 3C and fourth segment of video 3 in J Cell Biol. 2009 Dec 14;187(6):831-45.

Biological Sources
NCBI Organism Classification
Dendraster excentricus
Cell Line
Embryo
Cellular Component
microtubule
ensconsin
astral microtubule
cell cortex
cleavage furrow
Biological Context
Biological Process
mitotic anaphase
cytokinesis after mitosis
nocadazole treatment
Attribution
Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL15784
Archival Resource Key (ARK)
ark:/b7295/w9cil15784
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 768px 0.245µm
Y 768px 0.245µm
Time 10 seconds 81