The Cell: An Image Library

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CIL:152^*

Description

Phase contrast of a PtK1 cell (marsupial kidney epithelial cell) injected with Pak1 inhibitory fragment (H83L, 120 uM needle concentration) and X-rhodamine tubulin. Microtubules dynamics in these cells werevisualized using X-rhodamine. Image corresponds to Fig 4A and video 6 in J Cell Biol, 161:845-851, 2003. Video 6 is contained within the same image group as this figure.

Biological Sources

NCBI Organism Classification: Potorous tridactylus; (long-nosed potoroo)
Cell Type: epithelial cell
Cell Line: Pt K1 (NBL-3)
Cellular Component: cell; nucleus; middle lamella

Biological Context

Molecular Function: Pak1 inhibition

Attribution

Names: Torsten Wittmann; Gary M. Bokoch; Clare M. Waterman-Storer
Published: J Cell Biol, 161:845-851, 2003
Pubmed: 12796474

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1093px	——
Y	996px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.