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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:15005*  Cite 
Description

Distribution of interneurons expressing EGFP from the 5HT3 receptor promoter (Tg(Htr3a-EGFP)DH30Gsat, www.gensat.org) colabelled for calretinin in the cingulate cortex. A plexus of calretinin and EGFP positive axons can be seen in the superficial cortical layers. Many of the EGFP-expressing interneurons also express calretinin. Section (40 um) was imaged with a 32X, 0.4 NA Achroplan objective on a Zeiss Axiovert microscope. AF568 was excited at 562/40 nm and emission collected at 624/40 nm with Semrock filters and a single band dichroic (530-585 nm reflected, 601-800 nm transmitted). AF488 was excited at 482/35 nm and emission collected at 536/40 nm with Semrock filters and a single band dichroic (446-499 nm reflected, 513-735 nm transmitted). DAPI was excited at 350/50 nm and emission collected at 460/50 nm with a CLP400 dichroic (Chroma). Single images were captured for each channel using a Zeiss Axiocam MR CCD and pseudo-colored using Axiovision. See Davis and Puhl 2011 (Pubmed ID 21305052) for staining protocol. This image is part of a series characterizing EGFP expression from the 5HT3 promoter throughout the brain.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
interneuron
Cellular Component
neuronal cell body
axon
Attribution
Names
Margaret I. Davis
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL15005
Archival Resource Key (ARK)
ark:/b7295/w9cil15005
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1300px 0.3518µm
Y 1030px 0.3518µm