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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:14651*  Cite 

Thin section of Prochloron, a photosynthetic prokaryote showing adpressed thylakoid membranes. This image group includes freeze-fracture images showing the particle sizes and distribution on the thylakoid membranes. See also: Giddings TH, Withers NW, Staehelin LA. 1980 Supramolecular structure of stacked and unstacked regions of the photosynthetic membranes of Prochloron sp., a prokaryote. Proc Natl Acad Sci U S A. 77:352-356.

Technical Details

Prochloron was collected at Coconut Island, Oahu, Hawaii. Green colonies of didemnid , were collected from shallow waters. Green spherical cells, identified as Prochloron, were released from the ascidian Diplosoma virensascidians by gently pressing on the colonies. Material was prepared for thin sectioning as follows: Didemnid colonies containing Prochloron were fixed on site for 2 hr in 1.5% glutaraldehyde in sea water, rinsed, and stored in sea water for 3-4 weeks. Samples were then postfixed in 2% aqueous KMnO4 for 24 hr at room temperature, dehydrated with an acetone series, and embedded in Spurr's resin. Thin sections were stained with aqueous uranyl acetate and lead citrate, and examined in a JEOL 100C TEM at 20,000X magnification. Images were recorded on film and subsequently digitized.

Biological Sources
NCBI Organism Classification
Cellular Component
Biological Context
Biological Process
Thomas Giddings
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
electron density
Visualization Methods
lead salt
uranyl salt
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
microtome-sectioned tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 3385px 1nm
Y 4608px 1nm