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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:1435*  Cite 
Description

Rabbit psoas skeletal muscle fibers were blended in the presence of 5 mM MgATP to dissociate thick and thin filaments. The medium was then gently replaced with 0.3M KCl to dissociate thick filaments. Dissociated filaments were adsorbed onto mica flakes (Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95), quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Biological Sources
NCBI Organism Classification
Oryctolagus cuniculus
Cell Type
skeletal muscle cell
Cellular Component
cytoskeleton
Biological Context
Biological Process
skeletal muscle contraction
Molecular Function
ATPase activity, coupled to movement of substances
Attribution
Names
Ip, Wallace
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL1435
Archival Resource Key (ARK)
ark:/b7295/w9cil1435
Dimensions
Spatial Axis Image Size Pixel Size
X 4149px 0.00027µm
Y 2797px 0.00027µm