DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Compare to control image CIL# 13897. Image is Fig 5C bottom right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
Cells (MATa tem1::KanMX ura3::eGFP-CNM67–TEM1::URA3 kin4::TRP1-pGAL1-10-KIN4) were grown in rich medium containing 2% galactose/ 2% raffinose. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.
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