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Description
DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with galactose. Overexpression of Kin4 arrests cells in anaphase. Compare with control image CIL# 13895. Image is Fig 5C top right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.
Technical Details
Cells (MATa kin4::TRP1-pGAL1-10-KIN4) were grown in rich media containing 2% galactose/2% raffinose. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.
Biological Sources
- NCBI Organism Classification
- Saccharomyces cerevisiae
- (Baker's Yeast, Brewer's Yeast)
- Cell Line
- W303
- Cellular Component
- spindle pole body
Biological Context
- Biological Process
- mitotic cell cycle spindle orientation checkpoint
- protein phosphorylation
- Molecular Function
- ATP binding
- protein serine/threonine kinase activity
Attribution
- Names
- Mauricio Valerio-Santiago
- Fernando Monje-Casas
- Published
- J Cell Biol. (2011) 192: 599-614.
- Link
- http://jcb-dataviewer.rupress.org/jcb/...
- Pubmed
- 21321099
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- differential interference contrast microscopy
- Parameters Imaged
- optical path length gradient
- Source of Contrast
- boundaries between regions with different refractive index
- Processing History
- unprocessed raw data
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- whole mounted tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 621px | .0644μm |
| Y | 621px | .0644μm |
*CIL – Cell Image Library accession number. Please use this to reference an image.