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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13891*  Cite 
Description

3HA-Bfa1 (red) is now symmetrically localized to both spindle pole bodies (SPB) in cells with constitutive targeting of Tem1 to both SPBs in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Compare to control image CIL# 13890. Image is Fig 4C, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 4 include CIL# 13890, 13891, 13892, 13893.

Technical Details

Cells (MATa tem1::KanMX ura3::eGFP-CNM67–TEM1::URA3 bfa1::3HA-BFA1) grown in rich media with glucose were fixed for 15 min in 3.7% formaldehyde and 0.1 M potassium phosphate buffer, pH 6.4. Cells were then washed twice with 0.1 M potassium phosphate buffer, pH 6.4, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4/0.033 M citric acid, pH 5.9. Fixed cells were digested with 0.1 mg/ml zymolyase-100T (US Biological) and 1/10 volume of glusulase (PerkinElmer) at 30C for 15 min, washed once, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4/0.033 M citric acid, pH 5.9. Primary antibodies were anti-HA monoclonal antibody (HA.11; 1:500) and anti-tubulin (Abcam; 1:500). Secondary antibodies were: anti-mouse Cy3 (for HA) and anti-rat FITC (for tubulin). Cells were resuspended in DAPI (1 mg/ml). Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Line
W303
Cellular Component
nucleus
spindle pole body
cytoskeleton
cytoplasm
microtubule
tubulin complex
Attribution
Names
Mauricio Valerio-Santiago
Fernando Monje-Casas
Published
J Cell Biol. (2011) 192: 599-614.
Pubmed
21321099
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13891
Archival Resource Key (ARK)
ark:/b7295/w9cil13891
Grouping This image is part of a group.
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 187px 0.0642µm
Y 187px 0.0642µm