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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13878*  Cite 
Description

During late anaphase, Tem1 (green) localized to the spindle pole body (SPB) in cdc15-2 bfa1Δ cells grown at the restrictive temperature. DAPI (blue) and DIC (hidden) are also shown. Compare to image CIL# 13879. Image is Fig S4D, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S4 include CIL# 13877, 13876, 13879, 13878.

Technical Details

Cells (MATa tem1::TEM1-yEGFP::SpHIS5 bfa1::HIS3 cdc15-2) grown at 37C were fixed in 2.5% formaldehyde for 10 min, washed twice, and resuspended in 0.1 M potassium phosphate buffer, pH 6.4. Cells were then fixed for 10 min in 80% ethanol and resuspended in 1 mg/ml DAPI. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (Leica) and ImageJ software.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Line
W303
Cellular Component
spindle pole body
nucleus
cytoskeleton
cytoplasm
Attribution
Names
Mauricio Valerio-Santiago
Fernando Monje-Casas
Published
J Cell Biol. (2011) 192: 599-614.
Pubmed
21321099
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13878
Archival Resource Key (ARK)
ark:/b7295/w9cil13878
Grouping This image is part of a group.
Sample Preparation
Methods
formaldehyde fixed tissue
ethanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 218px 0.0642µm
Y 218px 0.0642µm