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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13755*  Cite 

Hedgehog (Hh, red) is transcribed in apical cells of the Drosophila wild-type germarium (CIL# 13755 and this image). In the absence of boi (boi[e] mutant), Hh protein is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745), but Hh transcription is still in apical cells (CIL# 13754). Hh transcription is demonstrated by the Hh-lacZ reporter and follicle cells are highlighted using anti-Fas-3 (green). Image is Supplemental Figure S3A in J Cell Biol. 2010. 191: 943-952.

Technical Details

Fly ovaries were dissected and fixed as described previously (O’Reilly et al., 2008). Primary antibodies were anti-betagalactosidase and 1:25 mouse anti-Fas3 (Developmental Studies Hybridoma Bank [DSHB]. Secondary antibodies used were either FITC, Cy3, and/or Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).

Tiffiney R. Hartman
Daniel Zinshteyn
Heather K. Schofield
Emmanuelle Nicolas
Ami Okada
Alana M. O'Reilly
J Cell Biol. 2010. 191: 943-952.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 512px 0.2728µm
Y 512px 0.2728µm
Z 1px 0.042µm