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Description
Expression of WT PINK1-YFP (green) does not induce mitochondrial translocation of mCherry-Parkin (red) in PINK1 knock-out mouse embryonic fibroblasts (PINK1 KO MEFs). Transfected PINK1 KO MEFs were treated with DMSO for 3 hrs. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Supplemental Figure S1c, top left row of J Cell Biol, 191: 933-942, 2010. Images in Supplemental Figure S1c include CIL#s 13725, 13726, 13727, 13728.
Biological Sources
- NCBI Organism Classification
- Mus musculus
- (house mouse)
- Cell Type
- fibroblast
- Cellular Component
- mitochondrion
- cytosol
- nucleus
- mitochondrial inner membrane
- mitochondrial outer membrane
Biological Context
- Biological Process
- mitochondrion degradation
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process
- response to stress
- protein phosphorylation
- intracellular protein kinase cascade
- protein K48-linked ubiquitination
- protein K63-linked ubiquitination
- Molecular Function
- ubiquitin-protein ligase activity
- zinc ion binding
- protein binding
- protein serine/threonine kinase activity
- ATP binding
- magnesium ion binding
- ubiquitin protein ligase binding
- calcium-dependent protein kinase activity
- C3HC4-type RING finger domain binding
Attribution
- Names
- Seok Min Jin
- Michael Lazarou
- Chunxin Wang
- Lesley A. Kane
- Derek P. Narendra
- Richard J. Youle
- Published
- J Cell Biol, 191: 933-942, 2010.
- Link
- http://jcb-dataviewer.rupress.org/jcb/...
- Pubmed
- 21115803
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- single-spot confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- EYFP
- mCherryFP
- Processing History
- brightness and contrast adjusted
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 512px | .1395μm |
| Y | 512px | .1395μm |
*CIL – Cell Image Library accession number. Please use this to reference an image.