Licensing
Description
Expression of R98F PINK1-YFP (green) induces mitochondrial translocation of mCherry-Parkin (red) in the presence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone), indicating that this PINK1 mutant is functional for inducing Parkin translocation when located on the outer michondrial membrane. The R98F mutation in PINK1-YFP partially inhibits proteolytic cleavage. Transfected HeLa cells were treated with CCCP (10 µM) for 1 hr. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4e, bottom row, with DMSO control in Fig 4e, top row of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724.
Biological Sources
- NCBI Organism Classification
- Homo sapiens
- (human)
- Cell Type
- endothelial cell
- Cell Line
- HeLa
- cervical carcinoma
- Cellular Component
- mitochondrion
- cytosol
- nucleus
- mitochondrial inner membrane
- mitochondrial outer membrane
Biological Context
- Biological Process
- mitochondrion degradation
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process
- response to stress
- protein phosphorylation
- intracellular protein kinase cascade
- protein K48-linked ubiquitination
- protein K63-linked ubiquitination
- Molecular Function
- ubiquitin-protein ligase activity
- zinc ion binding
- protein binding
- protein serine/threonine kinase activity
- ATP binding
- magnesium ion binding
- ubiquitin protein ligase binding
- calcium-dependent protein kinase activity
- C3HC4-type RING finger domain binding
- oxidative phosphorylation uncoupler activity
Attribution
- Names
- Seok Min Jin
- Michael Lazarou
- Chunxin Wang
- Lesley A. Kane
- Derek P. Narendra
- Richard J. Youle
- Published
- J Cell Biol, 191: 933-942, 2010.
- Link
- http://jcb-dataviewer.rupress.org/jcb/...
- Pubmed
- 21115803
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- single-spot confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- EYFP
- mCherryFP
- Processing History
- brightness and contrast adjusted
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 512px | .1860μm |
| Y | 512px | .1860μm |