Image is Fig. 4D in PMID: 20679433. HeLa cells were transfected to co-express p125A-mCherry and Sec31A-GFP. p125A colocalizes extensively with Sec31A; live cell time-lapse video microscopy showed that p125A (red) and Sec31A (green) positive structures are colocalized stably with each other over time, and undergo homotypic fusion. The vesicular structures of p125A-mCherry colocalize extensively with those of Sec31A-GFP over a period of at least 30 min. Cells were cultured on glass-bottom culture dishes, transfected with plasmid constructs of interest, and imaged using the Olympus Fluoview 1000 confocal microscope. 60X objective.