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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13576*  Cite 

Constituvely active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) blocks redistribution of Helix Pomatia Lectin (HPL) (green) from Golgi to ER in Src-deficient SYF fibroblasts cotransfected with active Src (SrcE-DsRed) (red). HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HPL indicates distribution of GalNac-Ts. Active Src is enriched at the Golgi upon expression of Arf1(Q71L)-GFP . Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100) prior to staining with Hoechst (blue) and Alexa 647-conjugated-HPL. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with either a 60x objective (U Plan Super Apochromatic, UPLSAPO; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 7E in J Cell Biol. 189: 843-858. 2010. Images in Fig 7 include CIL#s 13571, 13572, 13573, 13574, 13575, 13576, 13577.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
cervical carcinoma
Cell Line
Cellular Component
Golgi stack
integral to membrane
David J. Gill
Joanne Chia
Jamie Senewiratne
Frederic Bard
J Cell Biol. 189: 843-858. 2010.
PMID: 20498016
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence microscopy
Source of Contrast
distribution of a specific protein
distribution of epitope
compartmentalization of stain or label
Visualization Methods
Alexa Fluor 647
Alexa Fluor 594
primary antibody plus labeled secondary antibody
Helix pomatia agglutinin
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1024px 0.207µm
Y 1024px 0.207µm
Z 1px 13.95µm