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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13466*  Cite 

Kre2-GFP is not efficiently maintained in the Golgi apparatus when Pik1-mediated PtdIns4P synthesis (the Golgi-localized PtdIns 4-kinase in yeast is composed of the Pik1 catalytic subunit and a myristoylated calcium-binding protein, Frq1) ceases, implicating Pik1 signaling in retrograde trafficking of Golgi residents. In this image, Kre2-GFP is expressed in the isogenic control strain for the frq1-1 mutant, shifted to restrictive temperature of 37C for 1h. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4D 37C FRQ1 panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4D include CIL#s 13460, 13461, 13462, 13463, 13464, 13465, 13466, 13467.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cellular Component
Golgi apparatus
integral to membrane
vacuolar lumen
Golgi membrane
Christopher S. Wood
Karl R. Schmitz
Nicholas J. Bessman
Thanuja Gangi Setty
Kathryn M. Ferguson
Christopher G. Burd
J Cell Biol. 187: 967-975. 2009
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
constrained iterative deconvolution
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 264px 0.0663µm
Y 264px 0.0663µm
Z 10px 0.4µm