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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13444*  Cite 

Intracellular localization of GFP-GOLPH3 in a wild-type S. cerevisiae background grown at 37C. In wild-type cells incubated at 26C or 37C, GFP-GOLPH localizes to intracellular membranes more robustly than yeast GFP-Vps74 with lower cytosolic GFP signal and brighter Golgi puncta compared with GFP-Vps74–expressing cells. The Golgi-localized PtdIns 4-kinase in yeast is composed of the Pik1 catalytic subunit and a myristoylated calcium-binding protein, Frq1. Vps74 is essential to maintain glycosyltransferases in the Golgi and results in this study reveal a previously unrecognized PtdIns4P-binding site in Vps74/GOLPH3 family proteins and link Pik1 signaling to retention of Golgi-resident proteins. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1A GOLPH3/wild-type 37C panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 1A include CIL#s 13439, 13440, 13441, 13442, 13443, 13444, 13445, 13446, 13447, 13448, 13458, 13459.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cellular Component
Golgi apparatus
extrinsic to membrane
Christopher S. Wood
Karl R. Schmitz
Nicholas J. Bessman
Thanuja Gangi Setty
Kathryn M. Ferguson
Christopher G. Burd
J Cell Biol. 187: 967-975. 2009
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
constrained iterative deconvolution
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 302px 0.0663µm
Y 302px 0.0663µm
Z 11px 0.4µm