The nucleolus during mammary epithelial differentiation and early mammary tumorigenesis. Proliferating 3D cultures of MCF-10.B2 cells treated to activate ErbB2 were stained at day 20 with ANA-N antibody to detect nucleoli (green) and counterstained with DAPI to delineate nuclei (blue). The stack is presented as a projected image in Fig 1A (Day 20, ErbB2 of Meaburn and Misteli, 2008 J Cell Biol 180:39-50. The z-series was recorded with a Zeiss LSM 510 META confocal microscope with a 63x 1.4 NA objective lens.
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