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Description
Z-focal series through a HeLa cell stained for DNA (blue), Aurora B (green), and phospho-Ser10-histone H3(red). This image is the control for the Bod-1 depletion experiment CIL: 13372. Bod1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis.
Technical Details
Cells were fixed with 3.7% paraformaldehyde or methanol for 2 min at ?20°C. Mouse anti–α-tubulin DM1A (Sigma-Aldrich), and mouse anti–Aurora B antibody AIM-1 (BD Biosciences) were used at 1:500. Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories. Image corresponds to Figure 5A from JCB vol. 179 no. 2 187-197.
Biological Sources
- NCBI Organism Classification
- Homo sapiens
- (human)
- Cell Line
- HeLa S3
- Cellular Component
- chromosome, centromeric region
- histone 3
- spindle
- DNA
- Aurora B
- cytoplasmic chromosome
Biological Context
- Biological Process
- mitosis
- Aurora B activity
- chromosome organization
Attribution
- Names
- Iain M. Porter
- Sarah E. McClelland
- Guennadi A. Khoudoli
- Christopher J. Hunter
- Jens S. Andersen
- Andrew D. McAinsh
- J. Julian Blow
- Jason R. Swedlow
- Published
- JCB vol. 179 no. 2 187-197
- Link
- Journal of Cell Biology
- Pubmed
- 17938248
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- fluorescence microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- primary antibody plus labeled secondary antibody
- Processing History
- constrained iterative deconvolution
Sample Preparation
- Methods
- chemically fixed tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 359px | 0.0629μm |
| Y | 334px | 0.0629μm |
| Z | 57 | .2μm |
*CIL – Cell Image Library accession number. Please use this to reference an image.