The intracellular mobility of MeCP2 (a methylated DNA-binding protein) followed in a mouse fibroblast stably expressing GFP-MeCP2 through a time series of images captured by confocal microscopy after bleaching a 2 micrometer circular area of euchromatin (center, right). Fluorescence recovery after photobleaching (FRAP) is rapid, indicating that MeCP2 in euchromatin is highly mobile.
Images were obtained using a Zeiss 510 Meta confocal microscope with excitation at 488 nm and emission between 505 nm and 530 nm. An initial scan of the cell was followed by the bleaching of a 2 um diameter spot of euchromatin, and 60 successive images recorded at 5 s intervals to measure fluorescence recovery due to MeCP2 mobility.
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