High resolution image of a longitudinal section through one of the radial arms of the contractile vacuole complex displaying two rows of openings from the smooth spongiome into the collecting canal. Two microtubules separate the two rows of openings. The smooth spongiome forms a thick coat of membrane tubules around the canal and outside this is a reduced number of decorated spongiome tubules. TEM taken on 2/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X.The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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