Alternate header for print version

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12858*  Cite 

Immunoelectron microscopy of mammary gland tissue from lactating rats with medial cisternae of the Golgi apparatus indicated by immunogold labeling of MG-160, a 160 kDa membrane sialoglycoprotein specific to medial Golgi.

Technical Details

Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by incubation with an antibody to MG-160 (mouse monoclonal) followed by colloidal gold-conjugated secondary antibody (10 nm anti-mouse; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in distilled water. Samples were viewed in a Philips CM10 electron microscope, and images were collected digitally. See Ladinsky and Howell (2007) for more information on methods used.

Biological Sources
NCBI Organism Classification
Rattus rattus
Cell Type
mammary alveolar cell
glandular epithelial cell
milk secreting cell
Cellular Component
smooth endoplasmic reticulum
lipid particle
Golgi medial cisterna
Biological Context
Biological Process
lipid storage
Molecular Function
protein binding
Mark Ladinsky
Kathryn E. Howell
Ladinsky, MS and KE Howell (2007)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
distribution of a specific protein
Visualization Methods
uranyl salt
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
cryostat-sectioned tissue
formaldehyde fixed tissue
embedded tissue
methylcellulose embedded
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 3643px 21.1667µm
Y 4169px 21.1667µm