The Cell: An Image Library - Image CIL:12628

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CIL:12628^*

Description

Fluorescent image of a mouse oocyte labeled with an antibody to tubulin, which is shown localized in the cortex of the oocyte. The sample was fixed in paraformaldehyde for subsequent immunolabeling with an antibody to tubulin and a secondly antibody conjugated to Alexa 488, which labels the microtubules green. The oocyte was imaged using a Nikon Eclipse inverted microscope and the original image was deconvoluted using Nikon's Elements software.

Biological Sources

NCBI Organism Classification: Mus musculus; (house mouse)
Cell Type: oocyte
Cellular Component: cell cortex; microtubule cytoskeleton

Attribution

Names: Sabrina Lin; Prue Talbot

Imaging

Image Type: recorded image
Imaging Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Alexa Fluor 488; primary antibody plus labeled secondary antibody
Processing History: deconvolution

Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1600px	0.345μm
Y	1200px	0.345μm

*CIL – Cell Image Library accession number. Please use this to reference an image.