Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12618^* Cite

Description

Surface of a mouse oocyte This transmission electron micrograph shows the cortex of a mouse oocyte that was undergoing fertilization. The sample was fixed using glutaraldehyde and osmium tetroxide, embedded in plastic, sectioned, and stained with uranyl acetate and lead citrate. Microvilli are present on the oocyte's surface and the zona pellucida is visible surrounding the oocyte. The image was taken with a Phillips 500 transmission electron microscope. The scale bar is 1

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: oocyte
Cellular Component: cell cortex; microvillus; zona pellucida

Biological Context

Biological Process: fertilization

Attribution

Names: Prue Talbot

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12618
Archival Resource Key (ARK): ark:/b7295/w9cil12618

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt; lead salt
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2048px	0.0067µm
Y	2048px	0.0067µm