The Cell: An Image Library - Image CIL:12604

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CIL:12604^*

Description

High resolution image of a colchicine-treated cell, used in an attempt to disrupt its microtubules. We found that microtubules already formed are not disrupted in Paramecium multimicronucleatum. This open cytoproct shows the microfilaments around the retrieved membrane vesicles. The larger vesicles appear to be expanded ER as ribosomes can be seen on their cytosolic face. A long membrane tubule appears to be bound to a microtubular ribbon that may have its origin at the cytopharynx. TEM taken on 1/16/73 by R. Allen with Hitachi HU11A operating at 60kV. Neg. 8,100X.

Technical Details

The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cytoproct; cell cortex; microtubule

Biological Context

Biological Process: microtubule cytoskeleton organization

Attribution

Name: Richard Allen (University of Hawaii)
Link: http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: colchicine pretreatment; glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4958px	1.6nm
Y	5646px	1.6nm

*CIL – Cell Image Library accession number. Please use this to reference an image.