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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12600*  Cite 
Description

Scanning electron micrograph of a migratory mouse bone-marrow-derived fibroblast. This cell was found amidst a colony of NPC (Niemann-Pick Disease type C) mutant mouse mesenchymal stem cells isolated from the bone marrow. This sample was photographed at a magnification of 1,198x and an accelerating voltage of 10kV with a 12.7mm working distance. The cells were grown to confluence on Thermanox® coverslips and fixed with glutaraldehyde and osmium tetroxide, then critical point dried. This coverslip was imaged using an FEI Quanta 200 scanning electron microscope. Note the large lamellipodium and the long retraction fiber extending from the trailing edge of the cell. Scale bar = 10µm.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
bone-marrow-derived fibroblast
Cellular Component
cell
cell surface
lamellipodium
retraction fiber
Biological Context
Biological Process
migration
Attribution
Names
Anna Baccei
Lamis Jarvinen
Marian Rice
OTHER
Mount Holyoke College
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12600
Archival Resource Key (ARK)
ark:/b7295/w9cil12600
Imaging
Image Type
recorded image
Image Mode
scanning electron microscopy (SEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
glutaraldehyde fixed tissue
critical_point dried specimen
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.01µm
Y 882px 0.01µm