The Cell: An Image Library

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CIL:126^*

Description

Telomeres isolated from chicken erythrocyte nuclei following restriction endonuclease treatment, and imaged by transmission EM after uranyl acetate negative staining. Specimen was also labeled with immunogold using an anti-TRF1 primary antibody. Telomere chromatin adopts a characteristic looped conformation which likely aids in protection from the rapid degradation that occurs with free DNA ends.

Technical Details

Image recorded with FEI Tecnai 12 TEM at 100KV and recorded on 2048x2048 CCD with 2x binning. See also: T Nikitina and C L Woodcock 2004 Closed chromatin loops at the ends of chromosomes. J Cell Biol 166:161-165.

Biological Sources

NCBI Organism Classification: Gallus gallus gallus
Cell Type: erythrocyte
Cellular Component: chromosome, telomeric region; chromatin; nucleosome

Biological Context

Biological Process: chromatin assembly; chromatin organization
Molecular Function: structural constituent of chromatin

Attribution

Names: Christopher Woodcock; Tatiana Nikitina

Imaging

Image Type: charge coupled device (CCD)
Imaging Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: negative staining; gold; uranyl salt
Processing History: unprocessed raw image

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	0.65nm
Y	1024px	0.65nm

*CIL – Cell Image Library accession number. Please use this to reference an image.